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Lithographically Defined Micropost Arrays for Programmable Actuation and Interfacial Hydrodynamics

https://doi.org/10.1021/acsapm.1c01133

Brooks, Jacob T.; Cribb, Jeremy; Falvo, Michael R.; Superfine, Richard (December 2021, ACS Applied Polymer Materials)

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Vertical Light Sheet Enhanced Side-View Imaging for AFM Cell Mechanics Studies

https://doi.org/10.1038/s41598-018-19791-3

Beicker, Kellie; O’Brien, E. Timothy; Falvo, Michael R.; Superfine, Richard (December 2018, Scientific Reports)

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Microfluidic viscometry using magnetically actuated micropost arrays

https://doi.org/10.1371/journal.pone.0200345

Judith, Robert M.; Lanham, Bethany; Falvo, Michael R.; Superfine, Richard; Waigh, Tom (July 2018, PLOS ONE)

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Micro-elastometry on whole blood clots using actuated surface-attached posts (ASAPs)

https://doi.org/10.1039/c4lc01478b

Judith, Robert M.; Fisher, Jay K.; Spero, Richard Chasen; Fiser, Briana L.; Turner, Adam; Oberhardt, Bruce; Taylor, R. M.; Falvo, Michael R.; Superfine, Richard (January 2015, Lab on a Chip)

We present a novel technology for microfluidic elastometry and demonstrate its ability to measure stiffness of blood clots as they form. A disposable micro-capillary strip draws small volumes (20 μL) of whole blood into a chamber containing a surface-mounted micropost array. The posts are magnetically actuated, thereby applying a shear stress to the blood clot. The posts' response to magnetic field changes as the blood clot forms; this response is measured by optical transmission. We show that a quasi-static model correctly predicts the torque applied to the microposts. We experimentally validate the ability of the system to measure clot stiffness by correlating our system with a commercial thromboelastograph. We conclude that actuated surface-attached post (ASAP) technology addresses a clinical need for point-of-care and small-volume elastic haemostatic assays.
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